Guinea Pig IL-5 ELISA Kit
SKU: 69408486899

Guinea Pig IL-5 ELISA Kit

Sale price$343.80 Regular price$382.00
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Ships within 48 hours · Estimated delivery Jul 5 - Jul 10

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Description

Guinea Pig IL-5 ELISA KitThe Guinea Pig IL 5 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Guinea pig IL5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Guinea pig IL5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,

The Guinea Pig IL-5 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IL5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig IL5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig IL5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig IL5 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Exchange/Return Notes
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SKU: 69408486899

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