Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 4 - Jul 9
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human CRN ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a corin (CRN) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of corin (CRN) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Corin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Corin protein (CRN) belongs to the type II transmembrane serine protease family with a molecular weight of approximately 170-200 kDa. Its N-terminus is located inside the cell and has a transmembrane region. The extracellular region contains two curled structures (Frizzled, Fz), 8 repeats of low-density lipoprotein receptor structures (LDLR), a scavenger-like receptor structure (SR) and a conserved C-terminal protease (Protease) region. The protease domain has a conserved structure unique to trypsin-like proteases, including enzyme active groups containing histidine (H), aspartic acid (D) and serine (S). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.9 ★★★★★
Based on 1663 reviews
Sort
Product Reviews
★★★★★ 5
WORKS! LIGHT! EASY TO APPLY AND USE!
Took and trip and knew I would be out in the sun. I know this is for the face, but I used on my arms, legs and face. It is light and easy to apply. Not sticky and heavy as some sunblocks can be. I am very pleased with this purchase/find, as it works well and was a fabulous price for this tube.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 15, 2025
★★★★★ 5
Lightweight, Smooth, and Truly Unscented — My New Go-To Sunscreen
Size: 6 Fl Oz (Pack of 1), Style: SPF 50
This is the first time I tried this Banana Boat unscented sunscreen, and I’m genuinely impressed. One of the biggest things I look for in sunscreen is how it feels on the skin, and this one delivers—it’s not sticky at all and goes on very smooth. It’s easy to apply and absorbs quickly without leaving that heavy, greasy feeling.
I also really appreciate that it’s unscented. There’s no strong or unpleasant smell, which makes it much more comfortable to use daily.
I’ve used other brands before, like Neutrogena and some higher-priced options, but honestly, this one stands out. It performs just as well—if not better—at a more reasonable price.
Overall, this is a great sunscreen that I’ll definitely keep using. Highly recommended if you want something lightweight, smooth, and fragrance-free.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 25, 2026
★★★★★ 5
BEST sunscreen on the market
Size: 6 Fl Oz (Pack of 1), Style: SPF 50
This sunscreen is truly the best sunscreen on the market. I have now bought 4 bottles, and plan to purchase more. It is extremely milky, dissolves onto the skin perfectly, it feels like you arent wearing any sunscreen at all. I tell everyone I know about this product.
I also purchase the FACE spf which is even more smooth. I wish they sold this in a giant container, because I apply this everyday !!
This sunscreen has made it more accessible for me to apply sunscreen daily, it also has very minimal scent, and never burns your eyes.
Personally I will never buy another type of sunscreen again after this sunscreen.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 8, 2026
★★★★★ 5
Good for acne prone sensitive skin
Size: 3 Fl Oz (Pack of 1), Style: SPF 50
I am in my 30s with combo leaning oil and acne prone fair skin with pigmentation issues and I’m on prescription tretinoin every day. I sometimes use hydroquinone when my pigmentation issues flare up. I wear sunscreen every single day to protect my skin. It’s especially important since I’m on Tret. daily. Being that I have acne prone skin finding a sunscreen has always been a HUGE challenge for me. I’ve spent so much time on SkinSort and similar sites looking through sunscreen ingredients lists trying to find ones that won’t break me out. I’ve purchased PLENTY of k-beauty, j-beauty, EU sunscreens and Us sunscreens from Sephora and drugstore only for them to break me out. My skin is very sensitive/breakout prone to long ingredients lists, emollients, heavy textures, oils and lots of botanicals. I randomly stumbled upon this banana boat sunscreen here on Amazon and saw the short and fairly simple ingredient list that is low (not free of- but low) in occlusive ingredients in comparison to other sunscreens so I decided to order it. It has not broken me out and it feels nice. No alcohol in it, no irritation, no pimples so far. The tube is user friendly, the texture is light and pleasant and the sunscreen is affordable and accessible. I also appreciate how it’s water resistant so can be worn every day OR pool/beach. I highly recommend this to anyone with acne prone or sensitive skin if you are ok with US chemical sunscreens. I prefer chemical sunscreens over mineral sunscreens as mineral sunscreens break me out. This affordable banana boat in my opinion is WAY better for me personally than high end and expensive options that have tons of pore clogging ingredients. Yes even the popular k-beauty sunscreens break me out unfortunately. Everyone is different and anything has a potential to clog someone but for me this sunscreen is way more safe for my acne prone skin than any other sunscreen option I’ve come across. The majority of sunscreens are just packed with pore cloggers, but this banana boat one is minimal in comparison. The tube is travel size (tsa approved) but bigger than your standard 1.7oz sunscreen. This is nice for me because like I mentioned I wear sunscreen every single day and re-apply if I’ll be outside in the sun. While spending time outside I did not burn while wearing this sunscreen and I’m extremely fair skinned. I also did not notice any new pigmentation issues. The texture is nice, light and pleasant. It wears well under makeup. I normally wash my face in the AM, apply a hydrating kbeauty toner, tiny little dots of super light gel moisturizer (not much- I’m oily!) and then apply this banana boat sunscreen on top. Sometimes I wear makeup sometimes I don’t. It works both ways. I have not experienced any pilling. I have not noticed any scent with this sunscreen other than the sunscreen filters smell which isn’t too bad at all in comparison to other sunscreens I’ve tried. The sunscreen itself adds a touch of moisture but I don’t find it to be greasy on my oily skin. In the summer I will probably use a hydrating toner or serum (skip gel moisturizer) and then apply the banana boat sunscreen since I live in a warm climate. Anyways, this is a very nice textured non offensive not overly clogging (for me at least) Us chemical sunscreen that has been very unproblematic for me. I just apply a decent amount every AM and don’t even think about it. Like I mentioned if I’m out in direct sunlight (normally I’m not in the winter) I’ll reapply as needed. In the PM I use bioderma micellar water and then follow it with my Remedy brand face cleanser and it takes the banana boat off fine. I’m interested in trying the spray version (always spray decent amount into hand and then apply!) for my body in the summer. For now I apply this banana boat sensitive face to my face, neck, hands or whatever exposed area. If you tried the banana boat light as air face spf that one is a little more matte and dry but it’s also more pore clogging in both my experience and by ingredients lists. This banana boat sensitive has a bit of a different texture that’s lightweight and still not greasy but it’s more of a traditional light lotion. The banana boat light as air face one felt great but over time gave me more closed comedones. Needless to say I highly recommend this lightweight Us chemical sunscreen to anyone looking for a simple waterproof Us chemical sunscreen that is affordable and easy. Obviously no white cast as its chemical. Sorry for the long review but it might help someone out. Also I purchased this with my own money I am not a part of one of those product reviewing groups- whatever they are called. No shade to anyone lol.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 6, 2026
★★★★★ 4
Great
Size: 3 Fl Oz (Pack of 1), Style: SPF 50
The sunscreen itself is really good. It applies smoothly just as advertised “lotion”. I was pleased to see it didn’t leave a white cast. I apply makeup over my sunscreen often. This sunscreen worked perfectly with makeup. Like it said, it’s unscented and that was true as I didn’t detect a scent. My skin feels nice with this on. My only issue is that it didn’t come with a safety seal under the cap. Usually all products come with a seal to ensure it isn’t tampered with and that buyer gets the full amount of product. That was a bit upsetting but hopefully I didn’t actually get a used one.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 3, 2026
recommand products
University of Notre Dame Custom Blanket, Personalized Fighting Irish Indiana Graduation Gift
44.99
Harbour Side Chair, Black Swivel/Black Shell
252.00
Custom Bridal Shower Banner Tapestry, Personalized Floral Welcome Sign, Linen Wedding Backdrop
39.99
University of North Carolina Wilmington (UNCW) Personalized Blanket, Custom Seahawks Alumni Graduation Gift
89.99
Square grydelapper, Brun læder
35.90